Gryphon™ pCHAC Retrovirus
Retroviruses are considered the easiest and fastest means to deliver genes stably to mammalian cells. Allele has upgraded its retrovirus packaging from the previously offered Phoenix packaging system. The system provides either an Ecotropic packaging system (for dividing murine or rat cells) or an Amphotropic system (for dividing cells of most mammalian species, including human). We have upgraded our custom packaging method to a more efficient system that routinely results in a titer of 10^8 or higher. We have stopped offering the previously known Phoenix amphotropic and ecotropic cells. A similar Ecotropic and Amphotopic packaging cell line, Phoenix2, originated from different sources that are currently available.

• mWasabi; a green fluorescent protein as a transfection marker.
• Integrated Allele's engineered shRNA expression cassette for the highest efficiency of RNAi (US patents 7,294,504 and 7,422,896).

 

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Sapphire™ Baculovirus
The baculovirus expression system (BVES) provides a high-yield and low-cost platform for expressing functional eukaryotic proteins. Most eukaryote-specific post-translational modifications, such as signal cleavage, phosphorylation, glycosylation, and disulfide bond formation can occur in baculovirus-infected insect cells. Allele Sapphire™ Baculovirus DNA was specifically designed for efficient recombination with a polyhedrin promoter-based transfer vector by a simple co-transfection step.

• Virtually 100% recombination efficiency, which eliminates the time-consuming plaque purification step.
• A protein disulfide isomerase gene was inserted at the p10 locus of the virus to ensure proper protein disulfide bond formation.
• The Sapphire™ Baculovirus DNA is compatible with most of the commercial polyhedrin-based transfer vectors.

 

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Lentivirus/Retrovirus for Gene Expression
Allele´s Custom Service Division brought to the market advanced lentivirus and retrovirus packaging services. They are highlighted by a consistent high titer, large delivery viral counts, and a 100% success rate. Typically, lentiviruses are packaged by co-transfecting a number of helper plasmids and in most cases amounting to 10^6TU (transduction units)/ml which is considered a good titer yield. The proprietary packaging technologies that are used to perform Allele's virus packaging services are unique and highly efficient, yielding 10^8 to 10^9TU/ml; without any concentrating steps. With a concentration step, however, 10^9 to 10^10TU/ml can be achieved which is particularly useful for in vivo uses.

• Unbeatable Prices and bulk discounts
• Sample Infectivity Data and Warranty
• A rich collection of Lentiviral/Retroviral plasmids

Lentivirus/Retrovirus for RNA Interference
Effective delivery of RNAi reagents into cells remains a major barrier for achieving expected gene silencing results. Lentiviral or retroviral vectors provide the highest delivery rate into difficult-to-transfect cells, especially primary cells or in vivo tissues. Combined with the only patented Pol III promoter-driven shRNA or miRNA platform, Allele Biotech provides the most efficient RNAi service:

• Gene-to-Silence RNAi service, from design to virus.
• Sequence-to-Virus Full Service, from sequence to virus.
• DNA-to-Virus Packaging Service, from plasmid to virus.

Baculovirus & Insect Protein Expression
Allele Biotech (now including Orbigen) Custom Baculovirus Protein Expression Service offers the fastest and most cost-effective way for you to take advantage of the Baculovirus expression technology.

• Up to a 6-fold yield enhancement and fast turnaround time.
• Experienced technical staff with more than 15 years of experience.
• Modularized and customized service; Competitive pricing to fit your budget.

 

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Phase I: Cat# ABP-CS-PEBV001
Description:   Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into recombinant Baculovirus transfer vector. Verification of the subcloned gene by restriction digest and sequencing. Preparation of the recombinant vector DNA suitable for co-transfection with baculovirus DNA.

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Phase II: Cat# ABP-CS-PEBV002
Description: Cotransfection of recombinant Baculovirus transfer vector containing gene of interest with 3 different linearized Baculovirus DNAs. Recombinant clones will be identified and amplified. The customer will choose the best recombinant baculovirus for the protein expression.

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Phase III: Cat# ABP-CS-PEBV003
Description: Amplification of the chosen recombinant Baculovirus and titration of final high-titer stock by end point dilution

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Phase IV: Cat# ABP-CS-PEBV004
Description:   Amplification of the chosen recombinant Baculovirus to a high-titer stock of more than 1 x 108 pfu per ml Infection of three insect cell lines (Sf21, Sf9, and T.ni using the high-titer stock and with or without booster virus Cells are harvested at two different time points(72hr or 96hr)

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Phase V: Cat# ABP-CS-PEBV005
Description: Amplification of recombinant Baculovirus stock for protein production; Expression of recombinant protein under optimized conditions.

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Phase VI: Cat# ABP-CS-PEBV006
Description: Cell pellet harvested from 1 liter of insect culture will be lysed with our optimized reagents and protocol. Protein purification using appropriate affinity resin for 6xHis or GST-tagged recombinant proteins. Purification can be performed either under native or denatured conditions (as desired by the customer). SDS Page and Western Blot will be performed for quality testing.

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