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Cell Transformation Assay's from Cell Biolabs
Transformation of normal cells into neoplastic cells results in a population capable of proliferating independently of internal and external signals that normally restrain growth. The soft agar colony formation assay has traditionally been used to monitor ancorageindependent growth, employing 3-4 weeks of cell growth followed by manuel cell counting.
We have advanced the soft agar assay to eliminate tedious manual cell counting, allow high-throughput drug screening, and enable recovery of transformed cells for downstream analysis. These advances have also allowed us to develop a unique kit for the separation of clonogenic cancer cells from normal cells in heterogeneous solid tumors.
We offer a variety of assay formats:
- Our CytoSelect™ 96-Well Cell Transformation Assay, Standard Soft Agar combines a soft agar medium with a fluorescent dye to eliminate manual cell counting and speed results.
- The CytoSelect™ 96-Well Cell Transformation Assay, Soft Agar with Cell Recovery advances the technology even further. This assay uses a proprietary soft agar-based medium that allows transformed cells to be recovered after culture for further downstream analysis.
- For even higher throughput, choose our CytoSelect™ 384-Well Cell Transformation Assay which uses the proprietary medium found in our Cell Recovery Compatible assay. Due to small well size, it is not recommended to use this format for recovery of transformed cells.
CytoSelect™ 96-Well Cell Transformation Assay-Traditional Soft Agar Colony Formation
- Cell Transformation Assay Principle
The CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring malignant transformation where no downstream analysis is required. Transformed cells cannot be recovered; however, no manual cell counting is required.
With this assay, cells are incubated in a semisolid agar medium for 6-8 days, then solubilized, lysed and detected using CyQuant® GR dye in a fluorometric plate reader.
- Fast Results: 6-8 days vs. 21 days
- Plate Reader Convenience: Eliminates manual counting
- Versatile Format: Designed for 96-well throughput, but van be adapted for 48, 24, 12 or 6-well
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| CBA-130 | CytoSelect 96-well Cell Transformation assay | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
96 wells | € 535.00 | |
| CBA-130-5 | CytoSelect 96-well Cell Transformation assay | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
5 x 96 wells | € 2013.00 |
Recent Product Citations
CytoSelect™ 96-Well Cell Transformation Assays-Advanced Soft Agar with Post-Incubation Cell Recovery
- Cell Transformation Assay Principle. Cell colonies form after a 6-8 day incubation with agar matrix. Transformed cells can then be either lysed and detected with a fluorescent dye or recovered and re-plated.
The CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) provides a robust system for screening oncogenes and cell transformation inhibitors. Transformed cells may be recovered for further downstream analysis following colony formation.
- Faster Results: 6-8 days vs. 21 days
- Cell Recovery: Transformed cells remain viable for further analysis
- Plate Reader Convenience: Eliminates manual counting of cells
- Versatile Format: Designed for 96-Well through-put, but can be adapted for 48, 24, 12 or 6-well
- Easy Fluorescence Detection with the CytoSelect™ Cell Transformation Assay. HeLa and NIH3T3 cells were seeded at various concentrations and cultured for 6 days. Cell transformation was determined according to the assay protocol.
Product Name | Detection | Size | Catalog Number |
|---|---|---|---|
CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) | Colorimetric | 1 Plate* | CBA-135 |
CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) | Colorimetric | 5 Plates* | CBA-135-5 |
CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) | Fluorometric | 1 Plate* | CBA-140 |
CytoSelect™ 96-Well Cell Transformation Assay (Cell Recovery Compatible) | Fluorometric | 5 Plates* | CBA-140-5 |
CytoSelect™ 384-Well Cell Transformation Assay** | Fluorometric | 1 Plate *** | CBA-145 |
CytoSelect™ 384-Well Cell Transformation Assay** | Fluorometric | 5 Plates *** | CBA-145-5 |
* Each kit provides sufficient reagent quantities to perform 96, 48, 24, 12 or 6 test in a 96, 48, 24, 12 or 6-well plate, respectively.
** The 384-well kit does not allow for cell recovery due to small well size
*** Each kit provides sufficient reagents for one or five 384-well plates respectively.
