Endogenous miRNAs begin as long transcripts known as primary miRNAs (pri-miRNAs) which contain a hairpin structure containing the mature miRNA sequence. The hairpin structure, known as the miRNA precursor (pre-miRNA), is enzymatically excised and shuttled to the cytoplasm, where it is further cleaved to release the mature 18-24 bp miRNA.

We offer a large collection of premade microRNA expression vectors, each of which contains a sequence-specific pre-miRNA plus a selection marker.

If you don't see the microRNA you need, or if you're working with a species other than human or mouse, choose one of our miRNASelect™ Cloning and Expression Vectors and simply clone in your own precursor (pre-miRNA) sequence.

The miRNASelect™ Precursor Clone Collection contains over 600 expression vector constructs, each containing the full miRNA precursor (pre-miRNA) sequence to preserve putative hairpin structures. This ensures biologically relevant interactions that lead to proper cleavage into mature miRNAs.

Each miRNASelect expression vector also contains a selection marker:

• pEP vectors contain a puromycin selection marker
• pEGP vectors contain a GFP-puromycin fusion to allow selection by either marker.

Control vectors are available separately.