- Standard Curve Generated with the OxiSelect™ Protein Carbonyl ELISA Kit (STA-310).
OxiSelect™ Protein Carbonyl Assay Kits from Cell Biolabs
The most common products of protein oxidation in biological samples are the carbonyl derivatives of Pro, Arg, Lys and Thr residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species.
Our OxiSelect™ Protein Carbonyl Assay Kits provide rapid, efficient methods for detection of protein carbonyls. Three assays formats are available: immunoblot, ELISA or spectrophotometer. All formats are suitable for use with purified protein, plasma, serum, or cel lysate samples.
- Assay Principle for the OxiSelect™ Protein Oxidation Immunoblot Kit (STA-308).
Protein Carbonyl ELISA Kit
- Sensitive: Detects samples as low as 10 µg/ml
- Greater Sample Retention: No concentration or TCA precipitation steps that contribute to sample loss
Protein Carbonyl Immunoblot Kit
- No Molecular Weight shift: DNPH Derivatization after immunoblotting allows direct comparison of oxidized and non-oxidized protein fingerprints
Products
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| STA-308 | OxiSelect Protein Carbonyl Immunoblot Kit | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
10 blots | € 360.00 | |
| STA-309 | Oxidized Protein Immunoblot Control (Carbonyl-BSA) | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
10 ug | € 191.00 | |
| STA-310 | OxiSelect Protein Carbonyl ELISA Kit (1 plate) | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
96 wells | € 604.00 | |
| STA-310-5 | OxiSelect Protein Carbonyl ELISA Kit (5 plates) | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
5 x 96 wells | € 2253.00 | |
| STA-315 | OxiSelect Protein Carbonyl Spectrophotometric Assay (40 Assays) | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
40 assays | € 412.00 |
Assays for Lipid Peroxidation
Lipid peroxidation is a well-defined mechanism of cellular damage in both animals and plants that occurs during aging and in some disease states.
Our OxiSelect™ Lipid Peroxidation Assays allow you to quickly and easily quantify the most common markers and by-products of lipid peroxidation.
OxiSelect™ HNE-His Adduct ELISA kit
HNE (4-hydroxynonenal) is a well-known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress.
Our OxiSelect™ HNE-His Adduct ELISA Kit provides a simple, user-friendly way to assess HNE-His adduct formation in a variety of sample types.
- Fast: obtain results in about 4 hours
- Versatile: Suitable for use with cell lysates, serum, or plasma samples
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| STA-334 | OxiSelect HNE-His Adduct ELISA Kit | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
96 wells | € 604.00 | |
| STA-334-5 | OxiSelect HNE-His Adduct ELISA Kit | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
5 x 96 wells | € 2253.00 | |
| STA-335 | HNE-BSA Control | Cell Biolabs | Assays/RIA/ELISA/EIA/Complete kits | N/A |
100 ug | € 255.00 |
Selection Guide for Oxidative Stress Assays by Sample Type
Marker or Type of Damage | Sample Type | Products | ||||
|---|---|---|---|---|---|---|
Cells | Tissues | Blood | Urine | Other | ||
DNA / RNA Damage | ||||||
8-hydroxyguanosine (8-OHG) | x | x | x | x | Cerebrospinal fluid | STA-325 |
8-hydroxydeoxyguanosine (8-OHdG) | x | x | x | x | STA-320 | |
Abasic (AP) sites | x | x | STA-324 | |||
Double-strand DNA breaks | x | STA-321 | ||||
Comet Assay (general DNA damage) | x | STA-350, STA-351, STA-352, STA-353, STA-354, STA-355, STA-356 | ||||
UV DNA Damage | x | x | STA-326, STA-327, STA-328, STA-329, STA-322, STA-323 | |||
Lipid Peroxidation | ||||||
4-Hydroxynonenal (4-HNE) | x | x | x | STA-334, STA-335 | ||
8-iso-Prostaglandin F2alpha (8-isoprostane) | x | x | x | x | STA-337 | |
Malondialdehyde (MDA) | x | x | x | x | STA-331, STA-332, STA-333 | |
TBARS | x | x | x | x | STA-330 | |
Oxidative Protein Damage | ||||||
Protein Carbonyl Content (PCC) | x | x | x | STA-308, STA-309, STA-310, STA-315 | ||
3-Nitrotyrosine | x | x | x | STA-303, STA-304, STA-305 | ||
Advanced Glycation End Products (AGE) | x | x | x | STA-311, STA-313, STA-314, STA-316, STA-317 | ||
Advanced Oxidation Protein Products (AOPP) | x | x | x | STA-318, STA-319 | ||
Reactive Oxygen Species | ||||||
Intracellular ROS | x | STA-342 | ||||
In Vitro ROS | x | x | x | x | STA-347 | |
Hydrogen Peroxide | x | x | x | x | STA-343, STA-344 | |
Antioxidants | ||||||
Catalase | x | x | x | STA-341 | ||
Glutathione | x | x | x | x | STA-312 | |
Superoxide Dismutase | x | x | x | x | STA-340 | |
Oxygen Radical Antioxidant Capacity (ORAC) | x | x | x | Food samples | STA-345 | |
Hydroxyl Radical Antioxidant Capacity (HORAC) | x | x | x | Food samples | STA-346 | |
Total Antioxidant Capacity (TAC) | x | x | x | x | Food samples | STA-360 |
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Recent Product Citations
Recent Product Citations
- Mukhopadhyay, P. et al. (2010). CB1 cannabinoid receptors promote oxidative stress and cell death in murine models of doxorubicin-induced cardiomyopathy in human cardiomyocytes. Cardiovasc. Res. 85:773-784. (STA-334)
- Maki, R.A. et al. (2009). Aberrant expression of myeloperoxidase in astrocytes promotes phospholipid oxidation and memory deficits in a mouse model of Alzheimer's disease. J. Biol. Chem. 284:3158-3169. (STA-334)
- Jia, L. et al. (2007). Acrolein, a toxicant in cigarette smoke, causes oxidative damage and mitochondrial dysfunction in RPE cells: protection by (R)-alpha-lipoic acid. Invest. Ophthalmol. Vis. Sci. 48:339-348. (STA-308)
- Liu, Z. et al. (2007). Hydroxytyrosol protects retinal pigment epithelial cells from acrolein-induced oxidative stress and mitochondrial dysfunction. J. Neurochem. 103:2690-2700. (STA-308)
- Maity, P. et al. (2008). Indomethacin, a non-steroidal anti-inflammatory drug, develops gastropathy by inducing reactiveoxygen species-mediated mitochondrial pathology and associated apoptosis in gastric mucosa: A novel role of mitochondrial aconitase oxidation. J. Biol. Chem. 284:3058-3068. (STA-308, STA-310)
- Kang, K.A. et al. (2010). KIOM-4 protects against oxidative stress-included mitochondrial damage in pancreatic β-cells via its antioxidant effect. Evid. Based Complement. Altern. Med. 10.1093/ecam/neq007. (STA-310)
- Cho, H-Y. et al. (2009). Antiviral activity of Nrf2 in a murine model of respiratoryy syncytial virus (RSV) disease. Am. J. Respir. Crit. Care Med. 179:138-150. (STA-310)
- Neretti, N. et al. (2009). Long lived indy induced reduced mitochondrial reactive oxygen species production and oxidative damage. PNAS 106:2277-2282. (STA-310)
- Paromov, V. et al. (2008). The infuence of N-acetyl-L-cysteine on oxidative stress and nitric oxide synthesis in stimulated macrophages treated with a mustard gas nalogue. BMC Cell Biology 9:33. (STA-310)
- Kim, S-H. et al. (2008). Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. Acta Pharmacol. Sinica 28(11):1319-1326. (STA-310)
- Lowry, M. et al. (2007). Lung lining fluid glutathione attenuates IL-13 induced asthma. Am. J. Respir. Cell Mol. Biol. 38(5):509-516. (STA-310)