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DyLight® conjugated Fluorescent Rabbit Monoclonal Antibodies

DyLight Fluorescent Dyes - High Intensity, Photostable

 

DyLight conjugated RabMAbs® provides the combined benefits of Epitomics Rabbit Monoclonal technology and highly functional DyLight fluorphores together for a high performance primary antibodies ideal for demanding Immunofluorescent and Flow Cytometry applications.

 

DyLight Dyes exhibit higher fluorescence intensity and photostability compared to traditionally available fluorescent dyes such as Alexa Fluor*, CyDye* and LI-COR* Dyes in various applications. Additionally, DyLight dyes remains highly fluorescent over a broad pH range (pH 4-9).

 

Vimentin RabMAb DyLight 488
Her2 RabMAb DyLight 488

 


Benefits of DyLight conjugated RabMAbs®

  • Bright fluorescence intense emission provides superior sensitivity and requires less conjugate
  • Excellent photostability exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions
  • Narrow emission spectra minimal bleed-through between fluorophore channels enables multi-color detection
  • Buffer stability DyLight fluorescent dyes are stable at broad pH range (pH 4-9)
  • High Affinity and Specificity of Rabbit Monoclonal strong specific signal with no or minimal background noise

 


DyLight conjugated RabMAb Products

Epitomics is continuing to expand their offering of conjugated antibodies.

 


DyLight Fluorescent Dye Properties

DyLight Fluors have absorption maxima ranging from 350nm to 777nm (Figure 1), covering the entire visible light spectrum and several key near-infared and infrared wavelengths. Both the absorption and emission properties of the DyLight Fluors match the output (excitation) and detection wavelengths of common fluorescence instrumentation (Table 1).
Please Note: While there are a range of dyes in different wave length available. Epitomics currently only offers 488 and 549 conjugated antibodies.

Fig 1. Emission spectra of DyLight Fluors 350-800. Line colors represent the approximate visible colors of the wavelength maxima.© 2010 Thermo Fisher Scientific Inc.
Table 1. Spectral Properties of DyLight Fluorescent Dyes.© 2010 Thermo Fisher Scientific Inc.

 


All DyLight products

Product Name Catalog # Clone ID Applications Species
β-catenin DyLight® 488 RabMAb® 4500-1 E247 ICC Hu
Akt1 (C-term) DyLight® 488 RabMAb® 4508-1 Y89 ICC,FC Hu
Androgen Receptor DyLight® 488 RabMAb® 4551-1 EP670Y FC Hu
Atg7 DyLight® 488 RabMAb® 4548-1 EP1759Y FC Hu
ATM DyLight® 488 RabMAb® 4503-1 Y170 ICC Hu
B7-2/CD86 DyLight® 488 RabMAb® 4512-1 EP1158Y FC Hu
Bad (N-term) DyLight® 488 RabMAb® 4509-1 Y208 FC Hu
CD20 DyLight® 488 RabMAb® 4504-1 EP459Y FC Hu
CD21 DyLight® 488 RabMAb® 4547-1 EP3093 FC Hu
CD38 DyLight® 488 RabMAb® 4550-1 EPR4106 FC Hu
CD56 / NCAM DyLight® 488 RabMAb® 4537-1 EP2567Y ICC,FC Hu
Cyclin B1 DyLight® 488 RabMAb® 4531-1 Y106 ICC,FC Hu
Cytokeratin 15 DyLight® 488 RabMAb® 4513-1 EPR1614Y ICC Hu
Cytokeratin 16 DyLight® 488 RabMAb® 4530-1 EP1615Y ICC Hu
FAK DyLight® 488 RabMAb® 4514-1 EP1831Y ICC,FC Hu
Filamin A DyLight® 594 RabMAb® 4549-1 EP2405Y ICC Hu
GAP43 (C-term) DyLight® 488 RabMAb® 4535-1 EP890Y ICC,FC Hu
GFAP DyLight® 594 RabMAb® 4544-1 EPR1034Y ICC Hu
Golgi Matrix Protein (GM130) DyLight® 488 RabMAb® 4533-1 EP892Y ICC Hu
GSK-3β (C-term) DyLight® 488 RabMAb® 4525-1 Y174 ICC Hu
Her2 DyLight® 488 RabMAb® 4527-1 EP1045Y FC Hu
Histone H3 Phospho (pS10) DyLight® 488 RabMAb® 4521-1 E173 ICC Hu
HMGB1 DyLight® 488 RabMAb® 4518-1 EPR3506 FC Hu
hTERT DyLight® 488 RabMAb® 4502-1 Y182 ICC Hu
JAK3 DyLight® 488 RabMAb® 4511-1 EP909Y FC Hu
JNK2 DyLight® 488 RabMAb® 4526-1 EP1595Y ICC,FC Hu
Ki-67 DyLight® 488 RabMAb® 4519-1 EPR3611 ICC,FC Hu
Ku80 DyLight® 488 RabMAb® 4517-1 EPR3467 ICC,FC Hu
Lamin A/C DyLight® 488 RabMAb® 4529-1 EPR4068 ICC Hu
Lamin A/C DyLight® 594 RabMAb® 4553-1 EPR4068 ICC Hu
MSH2 DyLight® 488 RabMAb® 4542-1 EPR3943 ICC,FC Hu
MSH6 DyLight® 488 RabMAb® 4541-1 EPR3945 ICC Hu
mTOR/FRAP DyLight® 488 RabMAb® 4510-1 Y391 ICC,FC Hu
N-Cadherin DyLight® 488 RabMAb® 4534-1 EPR1792Y FC Hu,Rt
NF-kappa-B p65 DyLight® 488 RabMAb® 4543-1 EP2161Y ICC,FC Hu
p21/Ras DyLight® 488 RabMAb® 4532-1 EP1125Y FC Hu
p53 (N-term) DyLight® 488 RabMAb® 4506-1 Y5 ICC Hu
p53 Acetyl (K382) DyLight® 594 RabMAb® 4545-1 EPR358(2) ICC Hu
PARP-1 (Cleaved p25) DyLight® 488 RabMAb® 4523-1 E51 ICC,FC Hu
PARP-1 (Cleaved p85) DyLight® 488 RabMAb® 4507-1 Y34 ICC,FC Hu
PCNA DyLight® 488 RabMAb® 4540-1 EPR3821 ICC Hu
PTEN (C-term) DyLight® 488 RabMAb® 4505-1 Y184 FC Hu
Rabbit IgG Control with DyLight® 488 conjugate RabMAb® 3054-1   ICC,FC  
Rabbit IgG Control with DyLight® 594 RabMAb® 3055-1   ICC,FC  
RUNX1 DyLight® 488 RabMAb® 4538-1 EPR3099 FC Hu
SNAP25 DyLight® 594 RabMAb® 4546-1 EPR3275 ICC Hu
SOX2 DyLight® 488 RabMAb® 4522-1 EPR3131 ICC Hu
Survivin DyLight® 488 RabMAb® 4520-1 EP2880Y ICC Hu
Tau DyLight® 488 RabMAb® 4536-1 EP2456Y ICC,FC Hu
TDT DyLight® 488 RabMAb® 4516-1 EPR2975 FC Hu
VEGFR-1 DyLight® 488 RabMAb® 4501-1 Y103 ICC Hu
Vimentin DyLight® 594 RabMAb® 4552-1 EPR3776 ICC Hu
Vimentin DyLight® 488 RabMAb® 4528-1 EPR3776 ICC,FC Hu
YBOX1 DyLight® 488 RabMAb® 4539-1 EP2708Y ICC Hu
ZAP70 (C-term) DyLight® 488 RabMAb® 4524-1 YE291 FC Hu
ZAP70 Phospho (pY292) DyLight® 488 RabMAb® 4515-1 EPR1073 FC Hu

 


* DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alexa Fluor® Dyes is a registered trademark of Molecular Probes Inc. (Life Technologies Corp.).
Cy-Dye® is a registered trademark of Amersham Pharmacia Biotech UK Ltd.
LI-COR® is a registered trademark of LI-COR Biosciences.

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