pTagGFP2-laminB1 is a mammalian expression vector encoding TagGFP2-lamin B1 fusion protein. The vector can be used for fluorescent labeling of lamin B1 in living cells. TagGFP2 codon usage is optimized for high expression in mammalian cells, i.e. humanized [Haas et al. 1996]. Human lamin B1 is fused to the TagGFP2 C-terminus. To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of TagGFP2-lamin B1 coding sequence [Kozak 1987]. pTagGFP2-laminB1 vector can be used as a source of TagGFP2-lamin B1 hybrid sequence. The vector backbone contains unique restriction sites that permit its excision and further insertion into expression vector of choice.

Catno: FP199
pTagGFP2-laminB1 vector 20 ug

Encyclo PCR kit is intended for most PCR applications. It is especially recommended for cDNA amplification because of optimal combination of high fidelity and processivity provided by Encyclo polymerase. Evrogen Encyclo polymerase mix produces high yields of PCR products from a wide variety of templates and displays following features:

• 5’>3’ DNA polymerase activity with high processivity 
• Proofreading 3’>5’ exonuclease activity 
• Automatic hot start 
• TA cloning compatibility

Encyclo buffer has been developed to facilitate the amplification of specific PCR products and to provide successful amplification of long DNA templates. Encyclo PCR kit includes a mix of high-purity deoxyribonucleotides as well as sterile PCR water. Control DNA template and primer mix enclosed can be used for positive control PCR reaction.

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Mint and Mint-Universal cDNA synthesis kits are designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA. cDNA prepared by these kits are flanked by known adapter sequences at the both ends and can be used for various applications including construction of cDNA libraries, virtual Northern blot, subtractive hybridization, array probe preparation.