GeneTex, Inc.
GeneTex, Inc startte zijn activiteiten in het begin van 1997 in San Antonio, Texas. Gebaseerd op de brede ervaring en expertise van hun oprichters, waren de activiteiten van het bedrijf initieel gericht op een gamma aan reagentia voor kanker onderzoek. De eerste producten van GeneTex waren gericht op borstkanker onderzoek en prognose. Meer recent zijn er producten bijgekomen die wetenschappers kunnen gebruiken bij het onderzoek naar de basis mechanismen die gemeenschappelijk zijn bij vele vormen van kanker. Deze producten omvatten immunologische reagentia die gebruikt worden om specifieke proteïnen en mechanismen te onderzoeken die meespelen bij de regulatie van de cel cyclus, DNA reparatie en replicatie.
Belangrijkste Productlijnen:
- Cel- en Weefsel Lysaten
- Primaire Antilichamen
- Proteïnen en Peptiden
- Secondaire Antilichamen
- Serum Producten
Meer informatie kunt u vinden op: www.genetex.com
TGF-Beta Pathway
TGF-β Pathway
The transforming growth factor beta (TGF-ß) superfamily is composed of more than 30 soluble dimeric cytokines. This superfamily can be divided into the TGF-ß subfamily, which includes TGF-ß, activin and nodal, and the BMP (bone morphogenetic protein) subfamily, which includes the BMPs, anti-Muellerian hormone (AMH) and growth-differentiation factors (GDFs). The receptors for TGF-ß proteins are formed by combinations of type I and type II transmembrane serine/threonine kinases. Upon stimulation by a TGF-ß ligand, these receptors autophosphorylate and recruit adaptor proteins, which transduce signals to the nucleus via the Smad family of proteins. TGF-ß proteins are synthesized as large precursor proteins that associate with other intracellular proteins to form latent TGF-ß complexes. The initiation of TGF-ß signaling requires the activation of latent TGF-ß through either proteolytic cleavage or biochemical modifications to the latent TGF-ß complex. TGF-ß plays a dual role in the development of cancer, acting as both a tumor suppressor and a promoter of cancer progression, metastasis and immune evasion. Additionally, members of the TGF-ß family (especially Nodal and BMP) play a critical role in early embryonic development, germ layer specification and regulation of stem cell fate. GeneTex offers a wide range of antibodies to study the TGF-β pathway.
This is a small selection of product in the TGF-β pathway. For more product use out search engine of go to genetex.com
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| GTX19442 | Mouse MAb [BDI111] to AKT (unmodified S473) | Genetex | Primary Antibodies | ELISA, WB |
50 ug | € 360.00 | |
| GTX28805 | Rabbit PAb to AKT | Genetex | Primary Antibodies | ELISA, IF, IHC-P, IP, PCC, TCC, WB |
100 ul | € 306.00 | |
| GTX107921 | Rabbit PAb to MAP3K5 (mitogen-activated protein kinase kinase kinase 5) | Genetex | Primary Antibodies | WB |
100 ug | € 301.00 |
Induced Pluripotent Stem Cells (iPSCs)
Oct3/4, Sox2,c-Myc and Nanog are key transcriptional factors that are highly expressed in pluripotent cells and are down-regulated upon differentiation. These proteins form a transcriptional network to maintain the cells in a pluripotent state.
Two independent research teams reported that overexpression of these key transcriptional factors can induce pluripotency in terminally differentiated somatic cells.
Shinya Yamanaka and colleagues demonstrated the generation of induced pluripotent stem cells (iPSCs) from adult human fibroblasts with Oct3/4, Sox2, KIf4 and c-Myc (Cell 2007). Meanwhile, the laboratory of James Thomson reported that Oct3/4, Sox2, Nanog and Lin28 are sufficient to reprogram human somatic cells to pluripotent stem cells (Science 2007).
Easy iPSC Stem Cell Reprogramming… made easy!
Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells.
One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency.
The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
Cat. No. | Product | Includes |
|---|---|---|
GTX400001 | easy iPSC | 100 µL easy iPSC |
GTX400002 | easy iPSC + Antibody Sample Kit | 100 µL easy iPSC |
GTX400003 | easy iPSC + iPSC Enhancer | 100 µL easy iPSC |
Easy iPSC quickly induces pluripotency in target cells: GTX400001 easy iPSC
iPSC can be differentiated to any cell lineage
Easy iPSC Advantage
Comparison | easy iPSC | Plasmid | Virus |
|---|---|---|---|
Delivery | All genes in single transfection | One single or multiple plasmid | Multiple viruses delivered |
Safety | Extremely Safe | Extremely Safe | Requires BSL-2 |
Gene expression | Very High | Low | Very High |
Duration | Increased Duration | Extremely Short | Permanent |
Genomic Integration | No | No | Yes |
Stoichiometry | Predictable 1:1:1:1 | Complex or Unpredictable | Unpredictable |
Sample Preparation | Ready to Use | Requires isolation and purification | Requires tissue culture and purification |
Easy iPSC v.s. Plasmid
Easy iPSC's minicircle technology enable higher transfection efficiency and maintains expression at higher levels longer than transfection with plassmid DNA.
Easy iPSC v.s. Virus
Viruses cause transgenes to permanently integrate into the genome and risk insertional mutagenesis. easy iPSC allows for cells to be reprogrammed without integrating into the host cell genome. The southern blost shows multiple gene copies in virally-transduced cells.
Antibodies to iPSC Reprogramming Factors
Cat. No. | Product | Host & Clonality | Reactivity | Application |
|---|---|---|---|---|
GTX100863 | Nanog antibody | Rabbit pAb | Hu | ICC/IF, WB |
GTX109636 | c-myc antibody | Rabbit pAb | Hu | FACS, ICC/IF. WB |
GTX121923 | Lin28A antibody | Rabbit pAb | Hu | FACS, WB |
GTX101508 | Klf4 antibody | Rabbit pAb | Hu | FACS, WB |
GTX101507 | SOX2 antibody | Rabbit pAb | Hu | FACS, ICC/IF, IHC-P, WB |
GTX101497 | OCT3/4 antibody | Rabbit pAb | Hu | FACS, ICC/IF, IHC-P, WB |
