Reporter Cell Lines
Stable and Reporter Cell Lines allow researchers to quickly grow cells and begin their work, avoiding the time consuming and laborious task of transfecting and selecting stable lines themselves. Imgenex stable cell lines offer the most optimal and advanced systems available today.
TLR/HEK 293 Stable Cell Lines
Imgenex, introduces a complete and extensively validated set of human Toll-like Receptor (TLR) Cell Lines: HEK 293 cells stably transfected with unique and original plasmids for human TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, and TLR9. Designed for studying TLR expression and functional analysis, each TLR cell line has been validated with extensive functional testing. These products are the most optimized and advanced cell lines currently available.
Features
- Optimized TLR Expression
- Extensive Functional Validation
- Flexible and Advanced Research Tools
- Convenience
Products
TLR/NF-kB/SEAPorter™ HEK 293 Cell Lines
Imgenex, introduces SEAPorter™ Cell Lines: HEK 293 cells stably co-transfected with human Toll-like Receptor (TLR) and NF-kB reporter genes. Available are TLR2, TLR3, TLR4, TLR5, TLR7, and TLR8 and TLR9 cell lines. Stable TLR/NF-kB/SEAPorter™ HEK 293 cell lines are designed for sensitivity, broad working range dynamics and applicable for High-Throughput Screening for TLR agonists and antagonists.
Features
- Optimized TLR & NF-kB Expression
- Validated Cell Line Efficacy
- Fast
- Flexible
- High-Throughput
- Convenience
Products
NF-kB/SEAP Stably Transfected RAW Cells
- TLR ligand stimulation assay.
The RAW reporter stable cell line is a stably transfected RAW 264.7 cell line which expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. RAW 264.7 cells are known to respond to most Toll-like receptor (TLR) ligands, which trigger the NF-kB induction and lead to inflammatory cytokine production. RT-PCR tests also shows that RAW 264.7 cells produce all of the TLR mRNAs except for TLR5. Using a 96-well plate format assay, the RAW cell line has been validated by TLR ligand stimulation in which all of the TLR ligands except for TLR5 ligand activated the cell line.
Product
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| IML-120 | NF-kB/SEAP Stably Transfected RAW Cells | Imgenex | Cell lines/Competent cells | N/A |
1Vial | € 1055.00 |
LUCPorter™ Stably Transfected Cells
- Induction of IL-17A promoter activity
IL-17A Prom/LUCPorter™ HEK 293 is a stably transfected cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the IL-17A promoter. Induction of the IL-17A promoter by RORgt has been demonstrated (1) and also shown in our test (Figure 1). In the test, RORg or RORgt significantly mediated activation of the IL-17A promoter which was quantified by luciferase activity. Furthermore, Repression of the IL-17A promoter by a novel isoform of RORgt (RORgt[del. 5-8]), which lacks the hinge-encoding exons 5-8, has been demonstrated (2) and also shown in our test.
