Benefits of In-Cell ELISA technology:

• Fast and Simple - The assays are performed on cells which have been fixed in the microplate wells, eliminating the need for time-consuming preparation of cell lysates for Western blot analysis.
• Accurate - Not only is preparing samples for Western blot analysis time-consuming but it can also alter critical cell states such as enzyme phosphorylation. In-Cell ELISA Kits literally "freeze" the cells at the desired time point, eliminating further modifications from sample processing.
• Specific - All In-Cell ELISA-qualified antibodies have been stringently chracterized by mass spec and fluorescent imaging, and wherever possible by Western blot as well, to ensure that they are specifically binding to only the target enzymes.
• Reproducible - Because of its simplicity, In-Cell ELISA provides highly reproducible results, with c.v.'s under 10% easily achieved.
• Quantitative - Unlike Western blotting, which in even the best of cases will always be a qualitative approach, In-Cell ELISA provides data that is truly quantitative, as results can be quantified relative to total protein or to cell number.
• High-Throughput - Since the assays are designed for 96- or 384-well plates they offer unprecedented throughput potential as compared to Western blot, allowing for the testing of a vast number of treatments or other cell conditions. In-Cell ELISA also offers the potential to measure two different analytes plus total cell protein in the same well, providing true triplexing capability to further extend the analytical potential.
• Flexible - Each In-Cell ELISA Kit is offered in two formats, one of which utilizes infrared (IR) fluorescent dyes and one of which utilizes colorimetric detection. The IR kits are sometimes preferred for their improved dynamic range and slightly better reproducibility, but they also require a LI-COR® Odyssey® or Aerius® scanner. The colorimetric kits provide very comparable results to the IR kits but can be read by most standard plate readers.