Cancer is a broad group of diseases in which abnormal cells exhibit out-of-control growth. Cells become cancerous because of damage to DNA, regardless of whether such damage is genetically inherited or caused by environmental factors. Although most cancers form a tumor, some do not. Metastasis often occurs, in which cancer cells are disseminated throughout the body via lymph vessels or the bloodstream. Cancers are always named by their origin, even if metastasis has occurred. The study of genetic abnormalities found in cancer is a hot topic today. Two classes of cancer genes exist: oncogenes are cancer-promoting genes that are activated in cancer cells, whereas tumor suppressor genes are inactivated in cancer cells.
Spotlight on Dicer Knockout Embryonic Stem Cells
Dicer is an RNase III-containing enzyme that is central to the processing of most small RNAs. Deletion of Dicer in mouse embryonic stem cells results in severe proliferation and differentiation defects. A recent publication [PMID: 18923076] used Dicer knockout mouse embryonic stem cells as well as DGCR8 knockout mouse embryonic stem cells to studiy mammalian small RNA-generating pathways and shows that mammalian siRNAs exists in cell types other than oocytes.
These Dicer and DGCR8 knockout cell lines are valuable research tools for scientists interested in studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.
- Brightfield Image of Dicer knockout Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.
Featured Cell Line
Dicer knockout Mouse embryonic stem cells
Catalog# NBP1-96751
The Dicer knockout Mouse embryonic stem cells were derived from Dicer knockout mice. Dicer is essential for the processing of small non-coding RNAs including microRNAs and endogenous small interfering RNAs. These cells are a valuable research tool for scientists interested in studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.
- Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.
Additional Mouse Embryonic Stem Cell Lines
DGCR8 knockout Mouse embryonic stem cells
Catalog# NBA1-19349
Novus Biologicals now offers a novel a line of DGCR8 knockout mouse embryonic stem (ES) cells to study the global role of microRNAs. Embryonic stem cells provide a tool for the study of the molecular mechanisms of early mammalian development. The DGCR8 cells have been genetically altered at the locus of the dgcr8 gene such that no functional DGCR8 protein can be produced, resulting in the global, but specific, loss of micro RNAs.
- Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing on a Mitomycin C treated MEF cell feeder layer.
v6.5 Mouse embryonic stem cells
Catalog# NBP1-41162
The v6.5 mouse embryonic stem cell line was established from cells derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo from a C57BL/6 X 129/sv cross and has been used successfully in producing germline transmitting chimeras. The v6.5 mouse ES cell lines is the parent strain from which the DGCR8 -/- knockout cell line (NBA1-19349) is derived. Cells are at passage 20.
Cancer Antibodies
New Cancer Antibodies
- -Nodal Antibody Rabbit Polyclonal Catalog # NBP1-71686 Reacts with: Hu, Mu, Rt Works In:ICC, IF, WB ICC staining with in HeLa cells.
- -Nanog Antibody Rabbit Polyclonal Catalog # NB100-58842 Reacts with: Mu Works In: ICC, IHC-P IHC stain of Nanog in FFPE mouse testis
- -LAMP2 Antibody Rabbit Polyclonal Catalog # NBP1-71692 Reacts with: Bv, Hu, Mu, Rt, Po Works In: ICC, IF, WB IF staining at 1:200 in HepG2 cells.
- -Thrombin Receptor (N2-11_Antibody Mouse Monoclonal) Catalog # NBP1-71770 Reacts with: Hu, Mu Works In: ELISA, ICC IHC-P, WB IF stain in HeLa cells using FITC (green).
- -Thrombin Receptor (N2-11_Antibody Mouse Monoclonal Catalog # NBP1-71770 Reacts with: Hu, Mu Works In: ELISA, ICC IHC-P, WB IHC stain in FFPE mouse skin.
STK33 Antibody
Serine Threonine Kinase 33 is normally expressed in embryonic and germ-line cells, which ultimately differentiate into testis, lung, heart, brain and spinal cord. It is critical in both spermatogenesis and organ ontogenesis, and is thus a critical developmental protein. However, in many malignant tissues, STK33 is abnormally expressed, and its appearance in these tissues usually indicates an increase in the cancer cell viability. In cancer cells, STK33 suppresses mitochondrial apoptosis, which precludes normal processes to identify and eliminate cancerous cells.
CatNo |
Description |
Brand |
Category |
Application |
Amount |
Price |
Cart |
|---|---|---|---|---|---|---|---|
| NBL1-16551 | STK33 Lysate | Novus Biologicals | Tissue/Bodyfluids/Lysates | WB |
100 ug | € 275.00 | |
| NBP1-31602 | STK33 Antibody | Novus Biologicals | Primary Antibodies | WB |
100 ug | € 370.00 | |
| NBP1-32683 | STK33 Antibody | Novus Biologicals | Primary Antibodies | WB |
100 ul | € 370.00 |
SIRT1 Antibody
Sirt1 is a mammalian Class III HDAC that plays a significant role in the signaling pathways for autophagy, apoptosis, genome stability and transcriptional silencing. As a deacetylase, Sirt1 is known to interact with p53, FOXO, p65, Ku70 and possibly interacts with the full complement of Atg proteins including 5, 7 and 8. We do know that Sirt1 knockout mice are partially inhibited from producing autophagic vesicles under stress conditions and appear similar to Atg5 knockout mice, which experience early perinatal mortality. (Lee IH, et al. PNAS. 2008).
Request prices of SIRT1 Products at Bio-Connect
- IHC analysis of SIRT1 in human renal cell carcinoma using NBP1-49540
- WB analysis on 293 cell lysates using NBP1-40486.
- Position of the SIRT1 pre-design chimera RNAi. (H00023411-R01).
- Western blot analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529.
ApoE4 Antibody
ApoE variants have been implicated in the on-set of Alzheimer disease type 2. Specifically ApoE4 expression has been shown to be correlated with late on-set familial and sporadic forms of Alzheimer disease (AD). A greater number of ApoE4 alleles results in a heightened risk for AD, and decreases the mean age of onset. Recent studies have shown that ApoE4 preferentially depletes ApoER2, NMDA and AMPA receptors from the neuronal surface (Y Chen, et. al PMID 20547867 ). Depletion of these proteins facilitates B-amyloid synaptic suppression via inhibition of glutamatergic neurotransmission.
ApoE4 (4E4) Antibody - NBP1-49529 |
