Description:
HOT FIREPol^®Probe qPCR Mix Plus (ROX) is an optimized ready-to-use solution for real-time quantitative PCR assays with all the components necessary to perform qPCR: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and ROX dye according to system requirements. The user simply needs to add water, template, probe and primers. HOT  FIREPol^® Probe qPCR Mix Plus is compatible for use with most real-time thermal cyclers.

HOT FIREPol^® DNA Polymerase is inactive at ambient temperatures and therefore requires an icubation step at 95°C for 15 minutes prior to qPCR. This prevents the extension of nonspecifically annealed primers and primer-dimers being formed at low temperatures , during qPCR set-up. 

Features:

• High sensitivity with broad dynamic range
• Unique stability at ambient temperature
• Convenient ready-to-use solution
• Reaction set-up without using ice
• Available with ROX reference dye and without ROX
• Mixes compatible with most real-time thermal cyclers

Applications:

• Detection and quantification of DNA and cDNA targets 
• Profiling gene expression 
• Microbial detection
• Viral load determination

Quality control:

• Functional quality control via real-time PCR on different genomic templates
• Tests for stability at ambient temperature
• Tests for multiple freeze-thaw cycles

Description:
Hot FIREpol^® EvaGreen^® HRM Mix is an optimized ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen^® dye. It includes: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and ROX dye according to system requirements.

Features:

• Sensitive HRM genotyping
• Unique stability at ambient temperature
• Based on EvaGreen^® dye
• Convenient ready-to-use solution
• Reaction set-up without using ice

Quality control:

• Functional quality control via real-time PCR on different genomic templates
• Tests for stability at ambient temperature
• Tests for multiple freeze-thaw cycles