Favorgen Biotech Corp.
Favorgen is located in the national biotechnology park in Taiwan. Favorgen has invested substantially in its state-of-the-art manufacturing facilities, to provide customers with high-quality products in the Nucleic Acid Extraction field. They carry at the moment a very comprehensive product portfolio in this area, at very competitive prices. Favorgen is committed to developing research, both in-house and through collaborations with academic and industrial partners, in order to keep offering high-quality, innovative products.
Major Product Lines:
- Plasmid Extraction Kits
- DNA Fragment Extraction Kits
- Genomic DNA from Blood, Tissue or Plant
- DNA Isolation from Stool or Soil
- Total RNA Extraction from Blood, Tissue or Plant
- Extraction of Viral DNA/RNA
For more information, please visit: www.favorgen.com
Tri-RNA Reagent
Description
FavorPrep? Tri-RNA Reagent is a reagent from the improved phenol and guanidine isothiocyanate (GSN) method for the single-step RNA isolation. During sample homogenization or lysis, Tri-RNA reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. The composition of Tri-RNA reagent includes phenol and GSN in a mono-phase solution. A biological sample is homogenized or lysed in Tri-RNA reagent and homogenate is separated into the aqueous and organic phases by chloroform addition, vortexing,a nd centrifugation. RNA remains exclusively in the aqueous phase (clear upper phase), DNA in the interphase, and proteins remain in the organic phase (red color). RNA is precipitated from aqueous phase by addition of isopropanol and solubilized/concentrated. If needed, DNA and proteins can be sequentially precipitated from the interphase and organic phase with ethanol and isopropanol respectively, and solubilized/concentrated.
Features
- Single-step for the isolation of total RNA from tissues, cells, bacteria, plants, yeasts and biological fluids
- The entire procedure for total RNA isolation is less than 1 hour.
- The purified RNA can be applied in : RT-PCR, Northern hybridlation, RNase protection, Poly-A+RNA selection, Differential display, and Micro-array assay.
Applications
- For 100 applications
Storage
- At 4 ℃ in the brown glass bottle for routine use.
Procedure
- Add 1ml Tri-RNA Reagent to 100 mg tissue (or precipitated blood RNA viruses from up to 10 ml blood or 10 6 cultured cells or 10 cm 2 of culture plate)
- Homogenize tissue samples in Favorprep? Tri-RNA Reagent using a glass-Teflon or Polytron homogenizer (cultured cells can be lysed by repetitive pipetting; concentrated blood RNA viruses can be lysed by vigorous vortexing).
- Leave the homogenates for 5 minutes at room temperature.
- Add 0.2ml chloroform (not provided) and mix vigorously.
- Centrifuge at 12,000 rpm for 3 minutes to separate the phases, RNA is in the clear upper aqueous phase.
- Transfer the RNA phase to a clean tube.
- RNA is precipitated by adding 1x volume of isopropanol, vortex, leave at room temperature for 10 minutes, and then centrifuge at 12,000rpm for 15 minutes.
- Remove the supernatant.
- A brief spin to make sure the RNA pellet is precipitated to the designated side wall of the tube and then carefully remove any residue supernatant without touching the RNA pellet.
- Re-suspend the RNA in a small volume of TE, pH8.0
Ordering information
Cat. No. | Product Name | Package Size | Recommended Loading | Applications | Store at |
|---|---|---|---|---|---|
FATRE 001 | FavorPrep™ Tri-RNA Reagent | 100ml/bot | 1 ml | 100 | 4 ℃ (not frost-free) |
