HYPE-293™ Transfection Kit

HYPE-293™ Transfection Kit is dedicated to achieve High Yield Protein Expression in HEK-293, 293-S, 293-F or any 293-related cells. This transfection kit has been designed for maximum efficiency in HEK-293 cells growing in suspension. The transient transfection expression system represents an ideal and faster alternative to the stable expression system (costly and time-consuming). Scale-up to larger volumes for production of milligrams of protein per liter of cell culture is straightforward and easy with simple and cost efficient handling steps.

Main features are:
  • High protein & antibody production yield
  • Suitable for all HEK-293 cells growing in suspension
  • Compatible with any synthetic or regular media used for protein production
  • Ideal for bioreactor, spinner or flasks
  • Rapid, simple to scale up and ready-to-use
  • Free from animal sources
Applications
HYPE-293™ Transfection Kit has been designed for large scale up transient transfection and high protein expression such as antibody. The system is optimized for maximum efficiency in all 293 cells. It has been used and validated with cells from different origins (293-F, HEK-293, 293-S etc.) cultured in suspension (flask, spinner and bioreactor) used to produce proteins.

Please do not hesitate to contact us at techsupport(at)bio-connect.nl so we can give you all the supportyou need.

HYPE-293™ Kit is suitable for any synthetic or regular culture media. It has been tested with variouschemically defined media:

Medium
HEK293
FreeStyle™ 293
Expi293™
Pro293™
EX-CELL®





The results shown below have been obtained with 293s and 293-F cells growing in FreeStyle™ 293 (Life Technologies) culture medium. The maximum efficiency has been reached when HYPE-293 reagent was used in combination with the B293 reagent.

Efficient protein production is highly dependent on the cell model used. For instance, adaptation of cells to growth in suspension, culture medium and cell density are important parameters to obtain maximal protein yield. We advise optimizing experimental conditions (see instruction manual) in order to reach the best efficiency.

High production yield for antibody
The results presented indicate that HYPE-293™ Transfection Kit (HYPE-293 + B293) achieves high antibody production level and largely outperforms other commercially available transfection reagents in 293-F cells growing in suspension with chemically defined medium. After 6 days in culture more than 600 mg/L of antibody can be produced under standard bioproduction process (30mL scale).



Fig.1: IgG Rabbit production after transient transfection of 293-F cells with HYPE-293 kit in 30 mL cell culture volume.
Transfections were performed using 1.5 µg of plasmid DNA (antibody-expressing positive control vector, ThermoFisher) per mL of cell culture. 2 µL of HYPE-293 reagent per µg of plasmid was used. 293-F cells were cultured in 30 mL of medium in 125 mL shaken flasks. B293 (1X final) was added to the cells just after the addition of the complexes (10µL in 1 mL). Density of cell culture was 1x106 cells/mL the day of transfection. Day 3 and day 6 post-transfection, 500µL of supernatant was collected and IgG was measured with Easy-Titer Rabbit IgG Assay Kit (ThermoFisher).

Outperforms Competitors

The results presented below indicate that HYPE-293™ Transfection Kit (HYPE-293 + B293) achieves the highest protein yield in comparison to other commercially available transfection reagents in 293-F cells growing in suspension with chemically defined medium.



Fig.2: SEAP Expression with HYPE-293 Kit vs. other reagents.
SEAP was produced by transient transfection using HYPE-293 Transfection Kit, Free Style Max transfection reagent, LP2K, FHD, 293Fectin or IT-PRO transfection reagents according to manufacturer’s instructions. 293-F cells were cultured in 1 mL volume in a 24-well shaken plate Density of cell culture was 1x106 cells/mL the day of transfection. Day 3 post-transfection, 20µL of supernatants were collected and SEAP activity was measured using SEAP assay Kit (#SP00500).

Easy and efficient scale-up
The results indicate that protein production in 293-F suspension cells can be easily scaled up by using HYPE-293™ Transfection Kit. It has been used in volume up to 300 mL of cell culture without any protocol modification and protein yield production is even higher when compared to lower cell culture volume.



Fig.3: SEAP Expression with HYPE-293 Kit in increasing volume of culture.

Transfections were performed using 0.75 or 1.5 µg of plasmid DNA per mL of cell culture. Ratio of 2 µL of HYPE-293 reagent per µg of plasmid was used. Density of cell culture was 1x106 cells/mL the day of transfection.

Cat. No.
DescriptionBrand
HY29315
1.5 mL HYPE-293 + 5 ml B293
OZ Biosciences
HY29330
2 x 1.5 mL HYPE-293 + 2 x 5 ml B293
OZ Biosciences
HY293150
15 mL HYPE-293 + 50 ml B293
OZ Biosciences
HY293300
2 x 15mL HYPE-293 + 2 x 50ml B293
OZ Biosciences



Source:
Brochure: HYPE-293™ Transfection Kit Results

Related to:
Brands: OZ Biosciences