Xl10-Gold Competent Cells (search for the: 200315)

• Provide 20- to 30-fold higher transformation efficiency of ligated DNA
• Ideal for transformation of large plasmid DNA
• Produce larger primary libraries
• Fast growth generates large colonies

Stratagene’s XL10-Gold® ultracompetent cells* were created for transformation of large DNA molecules with high efficiency. These cells exhibit the Hte phenotype, which increases the transformation efficiency of large and ligated DNA molecules. In addition, the XL10-Gold strain allows blue-white color screening, permits cloning of methylated DNA and produces high-quality miniprep DNA. XL10-Gold ultracompetent cells are the host cells of choice for cloning experiments that require high transformation efficiencies.

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PfuTurbo DNA Polymerase (search for the: 600250)

• Special formulation of cloned Pfu DNA polymerase and the exclusive ArchaeMaxx® polymerase enhancing factor1
• Same high fidelity as Pfu DNA polymerase with more robust performance
• Enhances PCR product yields and throughput
• Amplifies longer targets than other high-fidelity enzymes
• Requires shorter extension times, fewer PCR cycles and less DNA
• Ideal for amplification of complex genomic DNA targets up to 19 kb and vector targets up to 15 kb
• Hot-start version available for enhanced specificity

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Serva DNA Stain G (search for the: 39803.01)

SERVA DNA Stain G is a safer alternative to traditional ethidium bromide stain for detecting nucleic acid in agarose gels. It is at least as sensitive as ethidium bromide and can be used in exactly the same way in agarose gel electrophoresis. SERVA DNA Stain G can be added to the gel solution, working dilution is 1:20.000 to 1:50.000.
SERVA DNA Stain G emits green fluorescence when bound to DNA or RNA. It has one fluorescence exication maximum at ca. 300 nm and another at ca. 450 nm when bound to nucleic acid. SERVA DNA Stain G allows visualization of DNA (>50 ng) in the agarose gel under visible light. This eliminates the need for exposure to UV light, which can nick and damage DNA.
SERVA DNA Stain G is non-carcinogenic and according to the AMES test it causes significantly fewer mutations than ethidium bromide.

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