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CytoGLO Annexin V-FITC Apoptosis Kit

The Annexin V Assay, a classical technique for detecting apoptosis, continues to be the most commonly used method for detecting apoptosis by flow cytometry. IMGENEX's CytoGLO™ Kit is the only Annexin V kit to include stabilized control cells for simple and fast compensation adjustment.
 
Advantages

  • Set compensation and validate assay with control cells
  • Avoid using precious sample for compensation adjustment
  • Proprietary control cell preparation ensures long-term stability
  • Kit also includes wash buffer, optimized binding buffer, and Propidium Iodide (PI)

Description

Cat. No.

Unit

CytoGLO Annexin V-FITC Apoptosis Detection Kit

10085K

100 Tests

 

 


Figure 1. During early apoptosis, the plasma membrane loses asymmetry causing PS to be translocated from the cytoplasmic face of the plasma membrane to the external face which can be detected using Annexin V. As apoptosis progresses and the plasma membrane becomes compromised, PI is used as an additional marker of apoptosis.


Figure 2. Control Cells (Cat No:10085-5) were stained or unstained as indicated using the CytoGLOTM Annexin V-FITC Apoptosis Detection Kit.


 

Figure 3. Adherent RAW cells were treated with 0.05 μg/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Accutase and stained with the CytoGLOTM Annexin V-FITC Apoptosis Detection Kit.


IMGENEX serves as a leading source of research products and expertise for studies of Apoptosis, and related pathways of Necroptosis and Autophagy for a variety of different platforms including Immunhistochemistry, Western Blotting, and Flow Cytometry.

For more information on our portfolio please refer to the links below.

Caspase Antibodies

Noxa,   SMAD3,   RANK,   RANKL

Caspase Inhibitors

Mitochondrial Extraction Kit

Caspase Antibody Sampler Kit

Serine Protease Activity Detection Kit

 

Check our Apoptosis (27), Necroptosis (28) & Autophagy (29) Pathways!

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