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Search results: SMTN

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Reddot Biotech
ReactivityHuman
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Reddot Biotech
ReactivityRat
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Product group Assays
Reddot Biotech
ReactivityHuman
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Reddot Biotech
ReactivityRat
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DLdevelop
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DLdevelop
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DLdevelop
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DLdevelop
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Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Product group Antibodies
Atlas Antibodies
Anti-SMTNHPA051778
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSMTN
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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4
Product group Antibodies
Atlas Antibodies
Anti-SMTNHPA058590
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSMTN
  • SizePrice
Western blot analysis of Smoothelin using anti-Smoothelin antibody (A04895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A375 whole cell lysates, Lane 6: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothelin antigen affinity purified polyclonal antibody (Catalog # A04895) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothelin at approximately 120KD. The expected band size for Smoothelin is at 99KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Reddot Biotech
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSMTN
  • SizePrice