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Search results: TMEM199

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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTMEM199
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetTMEM199
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Primate
TargetTMEM199
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Primate
TargetTMEM199
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Primate
TargetTMEM199
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTMEM199
  • SizePrice
Western blot analysis in human cell line CACO-2.
Product group Antibodies
Atlas Antibodies
Anti-TMEM199HPA027051
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTMEM199
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
Product group Antibodies
Sino Biological
  • SizePrice
Product group Molecular Biology
Sino Biological
  • SizePrice