Antibodies
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Primary Antibodies
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Immunohistochemistry
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Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot, use at 1:10-1:500. Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended. http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm Identifies an 82 and 70 kD protein by immunoprec
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Human
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Mouse
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Monoclonal
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IgG
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