Anti-Nuclei Antibody, clone 235-1

Overview:
Product group:	Antibodies
Category:	Primary Antibodies
Application:	Immunohistochemistry
Application note:	Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot, use at 1:10-1:500. Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended. http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm Identifies an 82 and 70 kD protein by immunoprec
Species:	Human
Host:	Mouse
Clonality:	Monoclonal
Isotype:	IgG

Properties:
	Research Use Only
UNSPSC:	12352203
Storage instructions:	Maintain for 1 year at -20-¦C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Scientific information:
Scientific info:	Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
Clone ID:	235-1

Additional information:
Synonyms:	MAB1281; Merck (Millipore)