Bio-Connect

CD20 antibody

Research Use Only
ORB385684
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
Price on request
Packing Size
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biorbyt
  • Product Name
    CD20 antibody
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flow cytometry: 2-5ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT FACS, IF, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SPM494
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
  • Reactivity
    Human
  • UNSPSC
    12352203