CD20 antibody
ORB385684
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
Overview
- SupplierBiorbyt
- Product NameCD20 antibody
- Delivery Days Customer10
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierFlow cytometry: 2-5ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT FACS, IF, IHC-P
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSPM494
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionThis antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
- ReactivityHuman
- UNSPSC12352203