ChIPAb+ Histone H2A.Z

Catalog number: 17-10048
Brand: Merck (Millipore)
Packing: 25 assays
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Price: € 456.00
Expected delivery time: 7 days
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Product specifications for - ChIPAb+ Histone H2A.Z

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Overview: 
Product group: Antibodies
Category: Primary Antibodies
Application: ChIP
Application note: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 ug of either Normal Sheep IgG or 1 ug of Anti-Histone H2A.Z and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of Histone H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Part No. 22-004) for a positive, and GAPDH Coding primers as a negative assay (Please see figures). Data is presented as
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Host: SH
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Clonality: Polyclonal
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  Research Use Only
UNSPSC: 12352203
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Storage instructions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Scientific information: 
Scientific info: H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
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Synonyms: 17-10048; Merck; Millipore