ChIPAb+ NFkappaB p65 (RelA)

Catalog number: 17-10060
Brand: Merck
Packing: 25 assays
Price: € 431.00
Expected delivery time: 7 days

Product specifications for - ChIPAb+ NFkappaB p65 (RelA)

Product group: Antibodies
Category: Primary Antibodies
Application: ChIP
Application note: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from serum starved, TNFalpha-treated (20 ng/mL, 30 min) 293 cells (~3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 ug of Normal Mouse IgG or 4 ug of Anti-NFkappaB p65 (RelA) and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of NFkappaB p65 (RelA) associated DNA fragments was verified by qPCR using ChIP Primers, I?Balpha promoter as a positive locus, and
Species: Human, Mouse, Rat
Host: Mouse
Clonality: Monoclonal
  Research Use Only
UNSPSC: 12352203
Storage instructions: Stable for 1 year at -20-¦C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20-¦C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20-¦C may cause glycerol containing solutions to become frozen during storage.
Scientific information: 
Scientific info: The transcription factor NFkappaB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFkappaB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFkappaB1 (p50 and its precursor p105), and NFkappaB2 (p52 and its precursor p100). NFkappaB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFkappaB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFkappaB is the most abundant in cells.
Additional information: 
Synonyms: 17-10060; Merck (Millipore)