ChIPAb+ TCF-4

Catalog number: 17-10109
Brand: Merck (Millipore)
Packing: 25 assays
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Price: € 456.00
Expected delivery time: 7 days
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Product specifications for - ChIPAb+ TCF-4

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Overview: 
Product group: Antibodies
Category: Primary Antibodies
Application: ChIP
Application note: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from HT29 cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ug of Normal Mouse IgG or 2 ug Anti-TCF-4 and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of TCF-4 associated DNA fragments was verified by qPCR using ChIP Primers, SP5 Promotor as a positive locus, and GAPDH promoter primers as a negative locus (Please see figures). Data is presented as
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Host: MO
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Clonality: Monoclonal
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  Research Use Only
UNSPSC: 12352203
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Storage instructions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Scientific information: 
Scientific info: TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
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Synonyms: 17-10109; Merck; Millipore