EIF2A antibody

Biorbyt

EIF2A antibody

16

WB: 1:500-1000, IHC-P: 1:50-100, IF/ICC: 1:20-50, IP: 1:20-50, FACS: 1:20-50 FACS, ICC, IF, IHC-P, IP, WB

Research Use Only

IgG

Rabbit polyclonal antibody to EIF2A. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a welldocumented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and MettRNAi and transfers MettRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2GDPeIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN and TNF alpha induces potent phosphorylation of eIF2 alpha at Ser51

-20°C

12352203