Bio-Connect

Lambda Light Chain Antibody / Lambda Antibody

Research Use Only
ORB386002
Biorbyt
ApplicationsFlow Cytometry
Product group Antibodies
Price on request
Packing Size
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biorbyt
  • Product Name
    Lambda Light Chain Antibody / Lambda Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT FACS, IF, IHC-P, WB
  • Certification
    Research Use Only
  • Clone ID
    N10/2
  • Isotype
    IgG
  • Scientific Description
    Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 3:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed). Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]
  • UNSPSC
    12352203