Rabbit Serum Against Human Ig Lambda Light Chain (Free )

Biorbyt

Rabbit Serum Against Human Ig Lambda Light Chain (Free )

10

Dot Blot, ImmunoElectrophoresis

DOT, IEP

Research Use Only

Polyclonal

Unconjugated

Rabbit

IgG

Precipitating polyclonal rabbit antiserum to free human Ig lambda chain. To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 ul of sample in a well with a diameter of 1 mm against 60 ul antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.

A pool of purified Bence Jones lambda proteins isolated from human urine. Freunds complete adjuvant is used in the first step of the immunization procedure.

Human

12352203