MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
VEGFR2 Polyclonal Antibody
BS-10412R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Overview
- SupplierBioss Antibodies
- Product NameVEGFR2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
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- C3a receptor antagonist therapy is protective with or without thrombolysis in murine thromboembolic stroke. Ahmad S et al., 2020 Jun, Br J PharmacolRead more
- Silencing of RBP-JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Luo Z et al., 2020 Jan, Mol Med RepRead more
- Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension. Bhagwani AR et al., 2019, PLoS OneRead more
- Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model. Xiao D et al., 2017 Oct 17, OncotargetRead more
- Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model. Wang L et al., 2017 Apr, PeptidesRead more