Horse Prostaglandin E2 ELISA Kit (PGE2)

Catalog number: MBS733875_48stripwells
Brand: MyBioSource
Packing: 48 strip Wells
Other sizes: Other sizes available, please contact us.
Price: € 539.00
Expected delivery time: 21 days
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Horse Prostaglandin E2 ELISA Kit (PGE2)

Product group: Assays
Category: ELISA / EIA
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Store all reagents at 2-8°C
Scientific information: 
Scientific info: This PGE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Horse PGE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!Principle of the Assay: PGE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGE2 antibody and an PGE2-HRP conjugate. The assay sample and buffer are incubated together with PGE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE2 concentration since PGE2 from samples and PGE2-HRP conjugate compete for the anti-PGE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.