Human Interleukin 18, IL-18 ELISA Kit

Catalog number: MBS704332_24stripwells
Brand: MyBioSource
Packing: 24 strip wells
Other sizes: Other sizes available, please contact us.
Price: € 348.00
Expected delivery time: 21 days
Quantity:
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Human Interleukin 18, IL-18 ELISA Kit

Overview: 
Product group: Assays
Category: ELISA / EIA
Target: IL18
Target description: interleukin 18
Properties: 
Molecular weight: 22326 Da
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Unopened test kits should be stored at 2 to 8°C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information: 
Scientific info: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gene ID: 3606
Swiss prot ID: Q14116
Additional information: 
Synonyms: IGIF; IL-18; IL-1g; IL1F4; interleukin-18; IFN-gamma-inducing factor; IL-1 gamma; iboctadekin; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma