Human Methemoglobin, MHB ELISA Kit

Catalog number: MBS701970_24stripwells
Brand: MyBioSource
Packing: 24 strip wells
Other sizes: Other sizes available, please contact us.
Price: € 348.00
Expected delivery time: 21 days
Quantity:
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Human Methemoglobin, MHB ELISA Kit

Overview: 
Product group: Assays
Category: ELISA / EIA
Properties: 
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Unopened test kits should be stored at 2 to 8°C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information: 
Scientific info: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MHB bound in the initial step. The color development is stopped and the intensity of the color is measured.