MBS701186 | Mouse anti-double stranded DNA antibody, IgG ELISA Kit

Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Mouse anti-double stranded DNA antibody, IgG ELISA Kit

Overview:
Product group:	Assays
Category:	ELISA / EIA

Properties:
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	41116158
Storage instructions:	Unopened test kits should be stored at 2 to 8°C upon receipt. Please refer to pdf manual for further storage instructions.

Scientific information:
Scientific info:	The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated dsDNA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated dsDNA and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-double stranded DNA antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Scientific information:

Scientific info:

The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated dsDNA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated dsDNA and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-double stranded DNA antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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Mouse anti-double stranded DNA antibody, IgG ELISA Kit

Product specifications for - Mouse anti-double stranded DNA antibody, IgG ELISA Kit