Mouse anti-double stranded DNA antibody, IgG ELISA Kit

Catalog number: MBS701186_24stripwells
Brand: MyBioSource
Packing: 24 strip wells
Other sizes: Other sizes available, please contact us.
Price: On Request
Expected delivery time: 21 days
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Mouse anti-double stranded DNA antibody, IgG ELISA Kit

Product group: Assays
Category: ELISA / EIA
Species: Mouse
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Unopened test kits should be stored at 2 to 8-¦C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information: 
Scientific info: The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated dsDNA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated dsDNA and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-double stranded DNA antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.