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Mouse anti-double stranded DNA antibody, IgG ELISA Kit
Catalog number:
MBS701186_24stripwells
Brand:
MyBioSource
Packing:
24 strip wells
Other sizes:
Other sizes available, please contact us.
Price:
On Request
Expected delivery time:
21 days
Quantity:
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General
Pictures
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product specifications for - Mouse anti-double stranded DNA antibody, IgG ELISA Kit
Overview:
Product group:
Assays
Category:
ELISA / EIA
Species:
Mouse
Properties:
Datasheet:
Datasheet
Research Use Only
UNSPSC:
41116158
Storage instructions:
Unopened test kits should be stored at 2 to 8-¦C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information:
Scientific info:
The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated dsDNA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated dsDNA and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-double stranded DNA antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.