% Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.,Estimated Z-primes of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nm to 3.125nM mixed with UBA1, UBE2L3, and TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
% Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.,Estimated Z-primes of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nm to 3.125nM mixed with UBA1, UBE2L3, and TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
Nedd4 E3 Ligase TR-FRET Kit
SBB-KF0056
Product group Chemicals
Overview
- SupplierSouth Bay Bio
- Product NameNedd4 E3 Ligase TR-FRET Kit
- Delivery Days Customer10
- CertificationResearch Use Only
- Hazard InformationNon-hazardous
- Scientific DescriptionSouth Bay Bios NEDD4 E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto NEDD4 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto NEDD4 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. - South Bay Bios NEDD4 E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto NEDD4 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto NEDD4 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
- Storage Instruction-20°C,-80°C
- UNSPSC12352200