PARP1 Chemiluminescent Assay Kit (384-well)

Catalog number: 80569
Brand: BPS Bioscience
Packing: 384 reactions
Price: On Request
Expected delivery time: 7 days

Product specifications for - PARP1 Chemiluminescent Assay Kit (384-well)

Product group: Assays
Category: Cell based assays
Subcategory: Characterization
Application note: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Storage instructions: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Scientific information: 
Scientific info: The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.