Pig Follicle-stimulating hormone, FSH ELISA Kit

Catalog number: MBS703786_24stripwells
Brand: MyBioSource
Packing: 24 strip wells
Other sizes: Other sizes available, please contact us.
Price: On Request
Expected delivery time: 21 days
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Pig Follicle-stimulating hormone, FSH ELISA Kit

Product group: Assays
Category: ELISA / EIA
Molecular weight: 74881 Da
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Unopened test kits should be stored at 2 to 8°C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information: 
Scientific info: Follicle-stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotropes in the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. FSH and Luteinizing hormone (LH) act synergistically in reproduction. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it, two of these make the full, functional protein. Its structure is similar to those of LH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHB), which confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours. Its molecular wt is 30000.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) conjugated monoclonal antibody preparation specific for FSH and incubated. Then substrate solution A and B are added to each well. Only those wells that contain FSH, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Swiss prot ID: P25440