Porcine N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit

Catalog number: MBS762829_48stripwells
Brand: MyBioSource
Packing: 48 strip Wells
Other sizes: Other sizes available, please contact us.
Price: € 417.00
Expected delivery time: 21 days
Typical Testing Data
Typical Standard Curve

Product specifications for - Porcine N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit

Product group: Assays
Category: ELISA / EIA
Datasheet: Datasheet
UNSPSC: 41116158
Storage instructions: Store at 4°C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20°C. Other components at 2-8°C). Stable for 12 months (if the entire kit is stored at-20°C).
Scientific information: 
Scientific info: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Porcine NTProBNP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Porcine NTProBNP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine NT-ProBNP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Porcine NT-ProBNP can be calculated.
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