Rat ghrelin, GHRL ELISA Kit

Catalog number: MBS703885_24stripwells
Brand: MyBioSource
Packing: 24 strip wells
Other sizes: Other sizes available, please contact us.
Price: On Request
Expected delivery time: 21 days
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Rat ghrelin, GHRL ELISA Kit

Product group: Assays
Category: ELISA / EIA
Target: Ghrl
Target description: ghrelin and obestatin prepropeptide
Molecular weight: 13176 Da
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Unopened test kits should be stored at 2 to 8°C upon receipt. Please refer to pdf manual for further storage instructions.
Scientific information: 
Scientific info: Ghrelin was originally discovered as a preprohormone that stimulates the release of growth hormone from the anterior pituitary. Recent studies have shown that ghrelin, in concert with other hormones, helps to control both appetite and energy balance. The synthesis of ghrelin takes place predominantly in epithelial cells of the stomach, although it is also produced in smaller quantities in the placenta, kidney, pituitary and hypothalamus. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release from the pituitary. It has an appetite stimulating effect, induces adiposity, and stimulates gastric acid secretion, and it is involved in growth regulation. Ghrelin is synthesized as a preprohormone (13kDa), then proteolytically processed to yield a 28 amino acid peptide (3kDa). Obestatin, an appetite suppressing peptide hormone, is also produced in the process. An interesting and unique modification is imposed on the ghrelin hormone during synthesis in the form of octanoic acid bound to one of its amino acids. This modification is necessary for biological activity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRP-Ghrelin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GHRP-Ghrelin. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHRP-Ghrelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHRP-Ghrelin. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gene ID: 59301
Swiss prot ID: Q9QYH7
Additional information: 
Synonyms: appetite-regulating hormone; ghrelin/obestatin prepropeptide; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide