sRANKL (total) Human ELISA (Osteoprotegerin Ligand)

Catalog number: RD193004200R
Brand: BioVendor
Packing: 96 wells
Price: € 561.00
Expected delivery time: 7 days

Product specifications for - sRANKL (total) Human ELISA (Osteoprotegerin Ligand)

Product group: Assays
Category: ELISA / EIA
Assay Sensitivity: 0,4 pmol/l
Assay Precision: Inter assay: n = 4, CV = 12,0%, Intra assay: n = 8, CV = 9,4%,
Assay Sample Type: serum and plasma (EDTA, citrate, heparin)
Assay Time: The total assay time is about 20 hours
Assay Detection Range: 0,5 - 32 pmol/l
  Research Use Only
UNSPSC: 41116158
Storage instructions: Store the kit at 2-8-¦C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Scientific information: 
Scientific info: sRANKL, receptor activator of nuclear factor (NF)-kappaB ligand (also: osteoprotegerin ligand, OPGL), is a part of the TNF superfamily with high similarity to other members of that protein species. (SwissProt Nr. O14788). Three isoforms are produced by alternate splicing, two type II membrane proteins (ISOFORM 1, 317 AA, and ISOFORM 3, 270 AA), and a secreted molecule (ISOFORM 2, 244 AA). ISOFORM 1 is identical to previously reported RANKL and possesses intracellular, transmembrane, and extracellular domains, ISOFORM 2 does not have the intracellular and transmembrane domains, and ISOFORM 3 does not have the intracellular domain. A soluble form arises by proteolytic processing from membrane isoforms. Although all forms are bioactive, the membrane-bound proteins seem to be the homeostatic forms, while the production of soluble RANKL signals pathological conditions. RANKL, RANK, and osteoprotegerin (OPG) have been identified as the key molecular regulation system for bone remodelling. RANKL is the main stimulatory factor for the formation of mature osteoclasts and is essential for their survival. Therefore, an increase in RANKL expression leads to bone resorption and bone loss. RANKL is produced by osteoblastic lineage cells and activated T lymphocytes. It activates its specific receptor RANK, which is located on osteoclasts and dendritic cells. The effects of RANKL are counteracted by OPG, which is secreted by various tissues and acts as an endogenous soluble receptor antagonist. Imbalances of the RANKL/OPG system have been related to the pathogenesis of Paget s disease, benign and malignant bone tumors, postmenopausal osteoporosis, rheumatoid arthritis, bone metastases and hypercalcemia. Several studies using animal models have shown that restoring the RANKL/OPG balance (e.g. by administering OPG) reduces the severity of these disorders. Indication - Postmenopausal and senile osteoporosis - Diseases with locally increased bone resorption activity - Paget s disease - Periodontal disease - Cardiovascular disease, arterial calcification - Inflammatory diseases - Immunological disorders - Arthritis - Oncology
Swiss prot ID: O14788
Additional information: 
Synonyms: RD193004200R; BioVendor
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