Vitamin B12 ELISA Kit

Arigo Biolaboratories

Vitamin B12 ELISA Kit

23

ELISA

0.3 ng/ml

1 h (RT/shaker), 20 min (RT/dark)

Research Use Only

Vitamin B12 as a trace element belongs to the biologi-cally important chelate formers. The basic unit con-sists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the ab-sence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12. The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sam-ple pretreatment) compared with a conventional microbiological assay (24 to 48 hours).

2°C to 8°C

41116133