The CRISPR/Cas9 complex

Genome Editing using guide RNAs

The recent discovery of the CRISPR/Cas9 complex has provided researchers an valuable tool to target and modify any genomic sequence with high levels of efficacy and specificity. The system, consisting of an RNA-guided nuclease (Cas9) and guide RNA (gRNA) complementary to a target sequence, allows for sequence-specific cleavage of target loci across the genome.

Applications

• Genome editing and engineering of model organisms
  Ø Gene correction
  Ø Gene replacement
  Ø Gene knockin/knockout
  Ø Other genome modification
• Gene correction for disease specific iPSC
• Gene/miRNA knockdown
• Synthetic biology applications
• Gene/Cell-based therapy

CRISPR/Cas9 versus TALENs and ZFNs

Advantages

• Easier to implement
• Ability to nick either or both DNA strands
• More frequent target sites
• Higher targeting efficiency

Disadvantages

• Possibly higher off target effect
  Can be alleviated by paired Cas9 nickases

Products

• CRISPR/Cas9-Knockout Plasmids from Santa Cruz »»
  Pool of three plasmids each encoding a target-specific gRNA for maximum KO efficiency
• HDR plasmids from Santa Cruz »»
  Specific DNA repair template for a double stranded break
• Cre Vector from Santa Cruz »»
  To excise the genetic material inserted during homology-directed repair

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Related to:
Brands: Santa Cruz Biotechnology
News: CRISPR/Cas9-Knockout Plasmids
Product groups: Genome Editing and Engineering