Biolaminin-521

Product specifications for - Biolaminin-521

Overview:
Product group:	Cell Culture
Category:	Matrices
Application note:	Culture of human embryonic and induced pluripotent stem cells (hESC and hiPSC), mesenchymal stem cells (MSC) and most anchorage-dependent progenitor cell types. Differentiation and maintenance of specialized cells, such as hepatocytes, cardiomyocytes and neurons.

Properties:
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	60103905
Storage instructions:	Repeated freeze/thaw should be avoided

Scientific information:
Scientific info:	Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific. Biolaminin 521 LN (LN521) is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal component-free stem cell culture system. LN521 enables efficient single-cell passaging of genetically stable and pluripotent stem cells without need of any apoptosis inhibitors for superior quality of your cells and studies. After birth, α5-laminins represent the most common laminins in the body and LN521 supports many diverse tissue cell types, such as cells from pancreas, vascular, nervous and muscular systems.

Scientific information:

Scientific info:

Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific.

Biolaminin 521 LN (LN521) is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal component-free stem cell culture system. LN521 enables efficient single-cell passaging of genetically stable and pluripotent stem cells without need of any apoptosis inhibitors for superior quality of your cells and studies.

After birth, α5-laminins represent the most common laminins in the body and LN521 supports many diverse tissue cell types, such as cells from pancreas, vascular, nervous and muscular systems.

Safety information:
MSDS:	MSDS

Additional information:
Synonyms:	Biolaminin 521 LN; Laminin 521; Laminin-11; LN521; Human Recombinant Laminin-521; BioLamina; Bio-Lamina

Rodin S, Antonsson L, Niaudet C, et al. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun. 2014;5:3195.

Rodin S, Antonsson L, Hovatta O, Tryggvason K. Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions. Nat Protoc. 2014;9(10):2354-68.

Laperle A, Hsiao C, Lampe M, et al. α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal. Stem Cell Reports. 2015;5(2):195-206.

Zhang D, Yang S, Toledo EM, et al. Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP. Sci Signal. 2017;10(493)

Lu HF, Chai C, Lim TC, et al. A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells. Biomaterials. 2014;35(9):2816-26.

Miyazaki T, Nakatsuji N, Suemori H. Optimization of slow cooling cryopreservation for human pluripotent stem cells. Genesis. 2014;52(1):49-55.

Bergström R, Ström S, Holm F, Feki A, Hovatta O. Xeno-free culture of human pluripotent stem cells. Methods Mol Biol. 2011;767:125-36.

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Product specifications for - Biolaminin-521