hPDE7A-HEK293 Cell Line

Catalog number: 60407
Brand: BPS Bioscience
Packing: 2 Vials
Price: € 9342.00
Expected delivery time: 7 days

Product specifications for - hPDE7A-HEK293 Cell Line

Product group: Cell Culture
Category: Cell lines
Application note: Useful to monitor cAMP and PDE7A signaling pathway activity and to screen activators or inhibitors of PDE7A.
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352207
Form supplied: aqueous solution containing DMSO
Storage instructions: Store in liquid nitrogen immediately upon receipt.
Scientific information: 
Scientific info: Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.
Swiss prot ID: Q13946
Additional information: 
Synonyms: 60407; BPS Bioscience