NF-kappaB Reporter (Luc) - HCT116 Cell Line

BPS Bioscience

NF-kappaB Reporter (Luc) - HCT116 Cell Line

7

Research Use Only

NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The firefly luciferase gene is controlled by 4 copies of NF-kappaB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kappaB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. The NF-kappaB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-kappaB signaling in response to stimulants such as the cytokines TNF and IL-1beta, pathogen-associated molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern (DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for establishing cell-based screens for inhibitors that target specific NF-kappaB stimulating molecules. This cell line can be further modified to allow investigation of downstream NF-kappaB activities as a result of targeted genetic mutation(s).

Liquid Nitrogen

41116155