Chemotaxis Assays

Cell Migration, Invasion & Wound Healing

  • Fully quantify chemotaxis with no manual cell counting
  • Measure chemotaxis in less than 6 hours with most cell types
  • Membrane inserts are uncoated to allow use with any chemoattractant
  • Colorimetric or fluorometric detection
Chemotaxis Assay Selection by Cell Type
Cell Type
Pore Size
Cell Type
Pore Size
Astrocytes
12 µm
Lymphocytes
5 µm
Cancer cell lines
8 µm
Macrophages
5 µm
Endothelial cells
8 µm
Monocytes
5 µm
Epithelial cells
8 µm
Neutrophils
3 µm
Fibroblasts
8 µm
Slow-moving cells
12 µm
Leukocytes
3 µm
Tumor cells
8 µm

Overview

Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their enviroment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases.

CytoSelect™ Cell Migration Assays are ideal for determining the chemotactic properties of cells. The kits utilize polycarbonate membrane inserts in either 24-well or 96-well plates. Membranes are available in 4 pore sizes to accommodate a variety of cell types and sizes:
  • d8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines
  • 5 µm pore size is ideal for monocytes / macrophages
  • 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes
  • 12 µm pore size is suitable for astrocytes and other large or slow-moving cells

 
Figure 1: CytoSelect™ Chemotaxis Assay Principle. Migratory cells move through the polycarbonate membrane toward a chemoattractant underneath the membrane inserts.
Figure 2: Migration of Human Fibrosarcoma HT-1080 Cells. Cells were seeded at 30,000 cells per well of a 24-well plate and allowed to migrate toward 10% FBS for 4 hours in either the presence or absence of 2µM Cytochalasin D. Migratory cells on the bottom of the polycarbonate membrane were stained (top) and quantified in a fluorescence plate reader (bottom).

Cat. No.
DescriptionAmount
CBA-100
CytoSelect 24-well Cell Migration (8 um), Colorimetric
12 assays
CBA-101
CytoSelect 24-well Cell Migration (8 um), Fluorometric
12 assays
CBA-102
CytoSelect 24-well Cell Migration (5 um), Fluorometric
12 assays
CBA-103
CytoSelect 24-well Cell Migration (3 um), Fluorometric
12 assays
CBA-104
CytoSelect 96-well Cell Migration (3 um), Fluorometric
96 assays
CBA-105
CytoSelect 96-well Cell Migration (5 um), Fluorometric
96 assays
CBA-106
CytoSelect 96-well Cell Migration (8 um), Fluorometric
96 assays
CBA-107
CytoSelect 24-well Cell Migration (12 um), Colorimetric
12 assays
CBA-108
CytoSelect 24-well Cell Migration (12 um), Fluorometric
12 assays



Source:
Website: Cell Biolabs

Related to:
Brands: Cell Biolabs
Product groups: Assays