Bio-Connect

5-Ethynyl-2-deoxyuridine (5-EdU) [61135-33-9]

Research Use Only
CLK-N001-5000
Jena Bioscience
CAS Number61135-33-9
Product group Chemicals
Estimated Purity≥98%
Molecular Weight252.23 g/mol
€ 3.548,00
5 g
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Overview

  • Supplier
    Jena Bioscience
  • Product Name
    5-Ethynyl-2-deoxyuridine (5-EdU) [61135-33-9]
  • Delivery Days Customer
    5
  • CAS Number
    61135-33-9
  • Certification
    Research Use Only
  • Estimated Purity
    ≥98%
  • Molecular Formula
    C11H12N2O5
  • Molecular Weight
    252.23 g/mol
  • Scientific Description
    5-EdU (5-Ethynyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging [1-5]. Presolski et al.[6] and Hong et al.[7] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
  • SMILES
    n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200