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Chemical Structure
Chemical Structure
Chemical Structure

5(6)-FAM N-succinimidyl ester [117548-22-8]

Research Use Only
CDX-C0011
Chemodex
CAS Number117548-22-8
Product group Chemicals
Estimated Purity>98%
Molecular Weight473.4
Price on request
Packing Size
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Overview

  • Supplier
    Chemodex
  • Product Name
    5(6)-FAM N-succinimidyl ester [117548-22-8]
  • Delivery Days Customer
    10
  • CAS Number
    117548-22-8
  • Certification
    Research Use Only
  • Estimated Purity
    >98%
  • Hazard Information
    Non-hazardous
  • Molecular Formula
    C25H15NO9
  • Molecular Weight
    473.4
  • Scientific Description
    Chemical. CAS: 117548-22-8. Formula: C25H15NO9. MW: 473.4. Synthetic. Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0. - Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.
  • SMILES
    OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352200