gamma-[(6-Azidohexyl)-imido]-ATP

Jena Bioscience

gamma-[(6-Azidohexyl)-imido]-ATP

5

Research Use Only

≥95%

C16H28N9O12P3 (free acid)

631.37 g/mol (free acid)

Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were gamma-[(6-Azidohexyl)-imido]-ATP (compound 6[1]) has been successfully used instead of gamma-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

O[C@H]1[C@H](n2cnc3c(N)ncnc23)O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)NCCCCCCN=[N+]=[N-])[C@H]1O

-20°C

12352200