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N6-Propargyl-adenosine

Research Use Only
CLK-N004-5
Jena Bioscience
Estimated Purity≥95%
Product group Chemicals
Molecular Weight305.29 g/mol
€ 490,00
5 mg
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Overview

  • Supplier
    Jena Bioscience
  • Product Name
    N6-Propargyl-adenosine
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Estimated Purity
    ≥95%
  • Molecular Formula
    C13H15N5O4
  • Molecular Weight
    305.29 g/mol
  • Scientific Description
    N6-propargyl-adenosine (N6pA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. N6pA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
  • SMILES
    c1(c2c(ncn1)n(cn2)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)NCC#C
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200