Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) is a powerful technique classically used for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits, transcription factors, or other regulatory or structural proteins bound either directly or indirectly to DNA. Successful ChIP requires high quality ChIP-validated antibodies that can specifically detect proteins associated with target regions of chromosomal DNA. Traditionally, endpoint and/or quantitative PCR (qPCR) are performed after ChIP to verify whether a particular DNA sequence is associated with the protein of interest. Using this classical approach, researchers can evaluate the interactions of the proteins of interest with a limited number of known target genes.

A history of innovation

Upstate®, now part of Merck, launched the first ChIP kits in the 1990s. Since then, Merck has introduced an extensive line of ChIP technologies with many advantages:

• Improved sample prep
• One-day protocol
• High throughput ChIP
• Genome-wide analysis
• ChIP for tissues
• Optimized, specialized protocols
• Automation compatibility
• ChIP-validated antibodies
• Protein A, G, and A/G magnetic beads
• Alternate detection methods


Magna ChIP™ kits

Cat. No.Description
17-10086EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit
17-10078EZ-Magna ChIP HT96 Chromatin Immunoprecipitation Kit
17-1001
Magna ChIP 2 Chromatin Immunoprecipitation Human Promoter 244K Microarray Kit
17-1002
Magna ChIP 2 Chromatin Immunoprecipitation Mouse Promoter 244K Microarray Kit
17-1000Magna ChIP 2 Universal Chromatin Immunoprecipitation DNA Microarray Kit
17-1004Magna ChIP 2 Universal Chromatin Immunoprecipitation DNA Microarray Quad Kit
17-610Magna ChIP A Chromatin Immunoprecipitation Kit
17-10085Magna ChIP A/G Chromatin Immunoprecipitation Kit
17-611Magna ChIP G Chromatin Immunoprecipitation Kit
17-20000Magna ChIP G Tissue Kit
17-10457Magna ChIP HT96 ChIP and Thermal Plate Set
17-10459Magna ChIP HT96 ChIP Plate Set
17-10077Magna ChIP HT96 Chromatin Immunoprecipitation Kit
17-10458Magna ChIP HT96 Thermal Plate Set
17-1010Magna ChIP-Seq Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit



ChIP validated antibodies (ChIPAb+™)

Antibody recognition in the context of chromatin can differ from other immunoassays. Avoid ChIP failure due to poor antibody performance by using ChIPAb+™ antibodies. To ensure reliable performance in your lab, each lot is individually validated and tested for ChIP.

ChIPAb+™ kits are more than just an antibody. Each set also includes a negative control antibody, plus control primers for amplifying a known, enriched locus to help you validate your results.

 

ChIPAb+™ trimethyl-histone H3 (Lys9)

ChiPAb+™+ trimethyl-histone H3 (lys9) (17-625): Sonicated chromatin from NIH 3T3 L1 cells was subjected to chromatin immunoprecipitation using either normal rabbit igG or Anti- trimethyl-histone H3 (lys9) antibody and the magna ChiP™ A Kit (17-610). Successful enrichment of trimethyl-histone H3 (lys9)-associated DNA fragments was verified by qPCR using primers flanking the mouse p16 promoter.

Cat. No.Description
17-615ChIPAb+ Acetyl-Histone H3
17-10050ChIPAb+ Acetyl-Histone H3 (Lys4)
17-658ChIPAb+ Acetyl-Histone H3 (Lys9) Purified
17-630ChIPAb+ Acetyl-Histone H4
17-10045ChIPAb+ Acetyl-Histone H4 (Lys5)
17-600ChIPAb+ CREB
17-10044ChIPAb+ CTCF
17-677ChIPAb+ Dimethyl-Histone H3 (Lys4)
17-648ChIPAb+ Dimethyl-Histone H3 (Lys9)
17-10034ChIPAb+ EED
17-663ChIPAb+ EED
17-603ChIPAb+ Estrogen Receptor alpha
17-662ChIPAb+ EZH2, clone AC22
17-608ChIPAb+ HDAC1
17-10048ChIPAb+ Histone H2A.Z
17-10054ChIPAb+ Histone H2B
17-10046ChIPAb+ Histone H3 (C-term)

Cat. No.Description
17-675ChIPAb+ Histone H3 (Unmodified Lys4)
17-643ChIPAb+ Monomethyl-Histone H3 (Lys27)
17-613ChIPAb+ p53
17-10131ChIPAb+ Phospho-CREB (Ser133)
17-685ChIPAb+ Phospho-Histone H3 (Ser10)
17-641ChIPAb+ REST
17-620ChIPAb+ RNA Pol II
17-10057ChIPAb+ SMRT
17-656ChIPAb+ Sox-2, clone 6F1.2
17-601ChIPAb+ Sp1
17-661ChIPAb+ SUZ12
17-10098ChIPAb+ TATA Binding Protein (TBP)
17-622ChIPAb+ Trimethyl-Histone H3 (Lys27)
17-10032ChIPAb+ Trimethyl-Histone H3 (Lys36)
17-614ChIPAb+ Trimethyl-Histone H3 (Lys4)
17-678ChIPAb+ Trimethyl-Histone H3 (Lys4)
17-10130ChIPAb+ Trimethyl-Histone H3 (Lys79)
17-625ChIPAb+ Trimethyl-Histone H3 (Lys9)


Chromatin immunoprecipitation: accessories

Cat. No.
Description
17-375
EZ-Zyme Chromatin Prep Kit
16-661
Magna ChIP Protein A Magnetic Beads
16-663
Magna ChIP Protein A+G Magnetic Beads
16-662
Magna ChIP Protein G Magnetic Beads
17-10071
Magna GrIP HT96 Rack (for 96 well plates)
20-400
Magna GrIP Rack (8 well)
LSKMAGS08
PureProteome Magnetic Stand

Magnetic beads

Magna ChIP™ magnetic beads with protein A, G, or A/G are optimized specifically for ChIP applications and are a rapid, reproducible, and efficient reagent for collecting immunocomplexes in ChIP assays. Unlike conventional agarose beads, Magna ChIP™ magnetic beads are rapidly moved to the side of a reaction vessel when exposed to a magnetic field, and significantly reduce the handling time and mechanical stress on target immunocomplexes.

EZ-Zyme™ chromatin preparation kit

• No sonication
• Mild and efficient fragmentation of chromatin
• Compatible with native ChIP

Magnetic racks for ChIP assays

Choose one of their magnetic racks for Magna ChIP™ assays: the classic Magna GrIP™ rack, the extra-strong, contoured PureProteome™ magnetic stands, or the new Magna GrIP™ HT96 rack, which is ideal for high throughput ChIP.


Related to:
Brands: Merck
Product groups: Antibodies, Arrays / Slides, Technique Specific Products