CRISPR/Cas9 Genome Editing Products

CRISPR/Cas9 mediated genome editing is a powerful technique that allows you to create knock-in, knock-out, gene up-regulation/repression, and mutations of any gene, in any cell, in a highly targeted manner.

Bio-Connect offers target-specific CRISPR/Cas9 Knockout (KO) Plasmids, CRISPR Double Nickase Plasmids, CRISPR/dCas9 Activation Plasmids and CRISPR Lenti Activation Systems for over 18,910 human and 18,340 mouse protein encoding genes. CRISPR/Cas9 KO Plasmids and CRISPR Double Nickase Plasmids enable the identification and cleavage of specific genes encoding a protein of interest thereby eliminating production of that gene product (protein). CRISPR/dCas9 Activation Plasmids and CRISPR Lenti Activation Systems activate endogenous gene transcription using a robust synergistic activation mediator (SAM) system. This exciting new technology is a useful tool for studying protein function and signaling pathways.



OZ Biosciences: CRISPR/Cas9 Genome Editing Transfection & Transduction Reagents


Successful CRISPR/Cas9 genome editing can be performed through diverse approaches (plasmids, mRNA, nuclease, viral delivery). Accordingly, efficient nucleic acid delivery (transfection or transduction ) represents a critical step for genome editing experiments. With more than 10 years of expertise in the development of transfection reagents, OZ Biosciences offers tailored transfection solutions for CRISPR/Cas9 technology.



Figure 1: Adapted transfection reagents for each CRISPR/Cas9 approach.

For generation of cellular models, Cas9 and the designed sgRNA (a chimeric RNA containing all essential crRNA and tracRNA components) can be introduced into the target cells. The type II CRISPR/Cas system only needs a single Cas protein that can be expressed into target cells by: (1) plasmid transfection, (2) direct delivery of the active Cas9 endonuclease, (3) transfection of mRNA encoding for Cas9 or (4) by viral vectors transduction.

Product Name
Molecule Vector
TechnologyApplications
PolyMag CRISPR
Plasmid DNA
Magnetofection
Primary and hard-to-transfect cells
Pro-DeliverIN CRISPR
Protein
Lipofection
All Cells
RmesFect CRISPR
mRNA
Lipofection
All Cells
ViroMag CRISPR
Virus
Magnetofection
All cells including primary and hard-to-transfect cells



Source:
Website: OZ Biosciences, Santa Cruz Biotechnology

Related to:
Brands: OZ Biosciences, Santa Cruz Biotechnology
News: CRISPR/Cas9 Genome Editing Transfection Reagents, CRISPR/Cas9-Knockout Plasmids
​Product groups: Genome Editing and Engineering