LentiBrite GFP-LC3 Lentiviral Biosensor

Catalog number: 17-10193
Brand: Merck
Packing: 1 Vials
Price: € 555.00
Expected delivery time: 7 days

Product specifications for - LentiBrite GFP-LC3 Lentiviral Biosensor

Product group: DNA / RNA / Vectors
Category: Plasmid / vector
  Research Use Only
UNSPSC: 41106600
Storage instructions: Storage and HandlingLentivirus is stable for at least 4 months from date of receipt when stored at -80-¦C. After first thaw, place immediately on ice and freeze in working aliquots at -80-¦C. Frozen aliquots may be stored for at least 2 months. Further freeze/thaws may result in decreased virus titer and transduction efficiency. IMPORTANT SAFETY NOTEReplication-defective lentiviral vectors, such as the 3rd Generation vector provided in this product, are not known to cause any diseas
Scientific information: 
Scientific info: Also available: LentiBrite GFP-p62 Lentiviral Biosensor!/b a href='http://www.millipore.com/catalogue/item/17-10224'Click Here/a) Also available: LentiBrite RFP-p62 Lentiviral Biosensor!/b a href='http://www.millipore.com/catalogue/item/17-10404'Click Here/a) Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infections. Members of the LC3 family play a key role in the maturation of the autophagosome, the central organelle of autophagy. LC3 precursors, diffusely distributed in the cytosol, are proteolytically processed to form LC3-I. Upon initiation of autophagy, the C-terminal glycine is modified by addition of a phosphatidylethanolamine to form LC3-II, which translocates rapidly to nascent autophagosomes in a punctate distribution. DNA constructs encoding fluorescent proteins fused to LC3 are widely employed for introduction into cells for monitoring autophagosome formation by fluorescence microscopy. EMD Millipore’s LentiBrite GFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types.
Additional information: 
Synonyms: 17-10193; Merck (Millipore)