EasyBlot

EasyBlot, finally a solution to masking by denatured IgG!

Immunoprecipitation (IP) and co-Immunoprecipitation (co-IP) assays often necessitate the use of the same, or same-host, antibodies for both the IP and western blot steps of these procedures. In these situations the target protein signal can be partially or completely masked on the western blot by secondary antibody detection of the denatured heavy and light chains of the IP antibody. GeneTex’s collection of EasyBlot secondary antibodies eliminates this problem by binding only to the non-denatured primary antibody used for the western blot while ignoring the denatured IgG chains of the IP antibody. In addition, GeneTex’s EasyBlocker reagent eliminates background signal caused by free Protein A or Protein G. Together, these reagents will help you to get the cleanest results on your IP/co-IP experiments.





Clear
Efficiently eliminates denatured IgG masking of your target protein’s signal.
Compatible
Works well for immunoprecipitations and co-immunoprecipitations using Protein A-, Protein G-, or Agarose-conjugated antibodies.
Comprehensive
Individual EasyBlot reagents to detect primary antibodies from rabbit, mouse, rat, goat or sheep hosts.
Convenient
Simple! Just replace your regular secondary antibody with the appropriate EasyBlot secondary antibody! For background caused by free Protein A or Protein G, try our EasyBlocker reagent available alone or in a convenient kit form combined with individual EasyBlot secondary antibodies.


Masking bands by denatured IgG

Cat. No.
DescriptionAmount
GTX221666-01
EasyBlot anti Rabbit IgG (HRP)
50 ul or 250 ul
GTX221667-01
EasyBlot anti Mouse IgG (HRP)
50 ul or 250 ul
GTX628474-01
EasyBlot anti Rat IgG antibody (HRP) [GT323]
50 ul or 250 ul
GTX628547-01
EasyBlot anti Goat IgG antibody (HRP) [GT221]
50 ul or 250 ul
GTX628906-01
EasyBlot anti sheep IgG (HRP)
50 ul or 250 ul

Masking bands by denatured IgG & background from protein A/G

Cat. No.
DescriptionAmount
GTX225856-01
EasyBlot anti-Rabbit IgG Kit (Optimized for Prot A/G)
50 ul or 250 ul
GTX225857-01
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G)
50 ul or 250 ul
GTX228909-01EasyBlot anti-Rat IgG Kit (Optimized for Prot A/G)
50 ul or 250 ul
GTX228910-01
EasyBlot anti-Goat IgG Kit (Optimized for Prot A/G)
50 ul or 250 ul
GTX228911-01
EasyBlot anti-Sheep IgG Kit (Optimized for Prot A/G)
50 ul or 250 ul

Background from protein A/G

Cat. No.
DescriptionAmount
GTX425858
EasyBlocker
12 g or 62 g



Related to:
Brands: GeneTex
Product groups: Antibodies